AdmiR3 – Unravelling the mysteries of adrenarche.
PI: Prof. Dr. med. Christa E. Flück Pandey
Collaborators: Dr. med. Tanja Zingg, Dr. med. Marco Janner, and PD Dr. med. Claudia Böttcher
Supported by SNF 320030_207893
Adrenarche is a major event in human sexual maturation, occurring in both boys and girls around 6-8 years of age. Stereotypical signs are the appearance of pubic and axillary hair, of adult body odor, and acne. At a cellular level, adrenarche corresponds to the formation of a new tissue within the adrenal cortex, named zona Reticularis, which is responsible for the production of androgens. However, how the zona Reticularis forms and what controls androgen production remain unknown. To fill this gap of knowledge, we have just kickstarted a non-interventional study comparing children in which adrenarche occurs prematurely (premature adrenarche, i.e. PA) with individual featuring timely adrenarche. Developmental stage-matched clinical observations on 30 prospective cases and 60 paired controls will be accompanied by non-invasive examinations, routine labs, and blood and urine collection, for the investigation of candidate markers and the identification of novel biomarkers (e.g. within circulating micro RNAs). Systematic data collection will be carried out at adrenarche, thelarche, menarche, and adulthood, over a period of more than 10 years. Prospective outcomes will serve a better understanding of the physiology of adrenarche and the plausible connection between PA and hyperandrogenic syndromes.
Connected projects:
Cholesterol metabolism and adrenocortical function (PI: Emanuele Pignatti, PhD)
Tracing novel androgen pathways in human fetal biology (PI: Therina du Toit, PhD)
miRNA signature in adrenarche (PI: Dr. med. Jani Liimatta). Funded by: Sigrid Jusèlius Foundation and the Foundation for Pediatric Research
Cholesterol metabolism and adrenocortical function.
PI: Dr. Emanuele Pignatti (ORCID 0000-0002-5372-5692),
Funding: NCCR RNA&Disease Translational Fellowship Grant (E.P.), IFCAH grant (E.P.), Novartis Foundation for Medical-Biological Research (E.P., 22B088), and UniBE Initiator Grant (E.P.).
Adrenocortical steroids are essential regulators of blood pressure, immune response, glucose homeostasis, stress control, and sexual maturation, and they are all synthetized from cholesterol. However, how cholesterol metabolism impacts steroid biosynthesis has not been explored. We found that intracellular cholesterol negatively affects the production of dehydroepiandrosterone, an androgen precursor that marks the onset of adrenarche (Pignatti et al., Endocrinology. 2022, 163(7), bqac076). Using animal models and human tissues, we also associated ABCG1, a cholesterol exporter, with negative regulation of glucocorticoid biosynthesis, and speculate about the modulation of this transporter as a potential clinical target for adrenocortical insufficiency (in press).
Tracing novel androgen pathways in human fetal biology
PI: Dr. Therina du Toit (ORCID 0000-0002-3533-0590).
Funding: Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2020, #101023999) and an UniBE Initiator Grant
This project aims to identify novel androgen metabolites and characterize their metabolic pathway in human fetal biology, with the specific focus on adrenal-derived 11-oxy androgens (11OxyAs). Steroidogenic enzyme expression in the fetal adrenal and the placenta hints at the production and metabolism of 11OxyAs in the fetal-placental unit. Thus, 11OxyAs are present in placental tissue, fetal cord blood, fetal serum and amniotic fluid, and could have a particular role during fetal development. The metabolism of the 11OxyAs in the fetal unit, therefore, presents as a focal point of investigation (du Toit T & Swart AC, J Steroid Biochem Mol Biol. 2021, 212, 105946). As adrenal androgens are primarily metabolized by the fetal liver cytochrome P450 3A7 (CYP3A7) producing metabolites that circulate to the placenta (Pignatti E, et al., Rev Endocr Metab Disord. 2023, 24, 5-21), the role of CYP3A7 in this biological route from the adrenal to the liver to the placenta is of interest, as it would regulate the biological activity of the 11OxyAs ―especially in clinical conditions characterized by adrenal androgen excess. Notably, CYP3A7 expression decreases after birth, with a concomitant increase in the expression of its isoform, CYP3A4 and, to date, the catalytic activities of these CYP3A isoforms towards the 11OxyAs have not been studied. The significance of this project is that the characterization of 11OxyAs and their downstream metabolites could prove integral to our understanding of the transition from the fetal to the neonatal stage and endocrine-related disorders that present at these stages. State-of-the-art high resolution mass spectrometry platforms, including LC-MS and GC-MS, are utilized to study steroid conversions and to identify steroid products (Andrieu T, et al., Anal Bioanal Chem. 2022, 414(25), 7461-7472).